Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human ERCC1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P07992
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
UV20, DNA REPAIR DEFECT UV-20 OF CHINESE HAMSTER OVARY CELLS, COMPLEMENTATION OF EXCISION-REPAIR, COMPLEMENTING DEFECTIVE IN CHINESE HAMSTER, excision repair cross-complementing 1 isoform 1, excision repair cross-complementing 1 isofrom 2, excision repair protein
Specificity Sensitivity:
ERCC1 antibody detects endogenous levels of total ERCC1 protein.
References:
Kan-Tai Hsia, Development, Jan 2003; 130: 369.
Jim Selfridge, Nucleic Acids Res., Nov 2001; 29: 4541 - 4550.
Cha-Kyung Youn, Cancer Res., Jul 2004; 64: 4849 - 4857.
Teresa A. Motycka, J. Biol. Chem., Apr 2004; 279: 13634 - 13639.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ERCC1 antibody (#C0184).
Western blot analysis of extracts from NIH/3T3 cells, using ERCC1 antibody (#C0184).