Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human Collagen IV.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P02462
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100ELISA: 1:20000
Alter Name:
COL4A1 NC1 domain, COLLAGEN OF BASEMENT MEMBRANE, ALPHA-1 CHAIN ARRESTEN, alpha 1 type IV collagen preproprotein, collagen IV alpha-1 polypeptide, collagen type IV alpha 1
Specificity Sensitivity:
Collagen IV antibody detects endogenous levels of total Collagen IV protein.
References:
Mary K. Chelberg, Cancer Res., Sep 1989; 49: 4796 - 4802.
Patricia L. Graham, J. Cell Biol., Jun 1997; 137: 1171.
PJ Lein, J. Cell Biol., Apr 1991; 113: 417.
EC Tsilibary, J. Biol. Chem., Dec 1988; 263: 19112 - 19118.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Collagen IV antibody.
Western blot analysis of extracts from HeLa cells, using Collagen IV antibody.