Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human APC.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P25054
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:40000
Alter Name:
AAPC; DP2.5; DP3; FPC; GS, ADENOMATOUS POLYPOSIS COLI, ATTENUATED, ADENOMATOUS POLYPOSIS OF THE COLON, APC GENE, DELETED IN POLYPOSIS 2.5, FAMILIAL ADENOMATOUS POLYPOSIS, FAMILIAL POLYPOSIS OF THE COLON, FAP GARDNER SYNDROME, POLYPOSIS, ADENOMATOUS INTESTINAL, adenomatosis polyposis coli tumor suppressor, adenomatous polyposis coli protein
Specificity Sensitivity:
APC antibody detects endogenous levels of total APC protein.
References:
T Maltzman, Carcinogenesis, Dec 1997; 18: 2435 - 2439.
Dhaval M. Patel, J. Immunol., Nov 1999; 163: 5201.
Qi Sun, J. Immunol., Oct 2000; 165: 4105
M Matsui, J. Immunol., Jul 1987; 139: 373.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using APC antibody.
Western blot analysis of extracts from J COLO205tcells, using APC antibody.