Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human AP-2.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05549
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
Alter Name:
AP-2 transcription factor, AP2A, AP2TF, TFAP2, TFAP2A, activating enhancer-binding protein 2 alpha, activator protein 2
Specificity Sensitivity:
AP-2 antibody detects endogenous levels of total AP-2 protein.
References:
ShunJiang Yu, J. Biol. Chem., May 2003; 278: 19597 - 19602.
TT Chen, Mol. Cell. Biol., Jun 1997; 17: 3056 - 3064.
Oscar B. Goodman, J. Biol. Chem., Oct 1995; 270: 23768.
Didier Jean, J. Biol. Chem., Jun 1998; 273: 16501.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AP-2 antibody.
Western blot analysis of extracts from COLO205 cells, using AP-2 antibody.