Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from human Actin.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P60709
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
Alter Name:
ACT, Actin, cytoplasmic 1
Specificity Sensitivity:
Actin-pan antibody detects endogenous levels of total Actin protein.
References:
Roxana Schillaci, J. Immunol., Mar 2006; 176: 3426 - 3437.
Maria Emanuela Cuomo, J. Biol. Chem., Oct 2005; 280: 35844 - 35858.
Wei Zhou, J. Clin. Endocrinol. Metab., Feb 2006; 91: 569 - 579.
Donna Brennan, J. Cell Sci., Mar 2007; 120: 758 - 771.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Actin-pan antibody.
Western blot analysis of extracts from HeLa cells, using Actin-pan antibody.