• Catalog Number:

  E1A0104L-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human Caspase 3.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42574

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein

• Specificity Sensitivity:

  Caspase 3 (cleaved-Asp175) antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

• References:

  John K. Jain, Biol Reprod, Aug 2005; 73: 382 - 388. C.S. Thota, Biol Reprod, Dec 2005; 73: 1191 - 1198. Koichi Ushizawa, Biol Reprod, Feb 2006; 74: 344 - 351. ULRICH STEIDL, Ann. N.Y. Acad. Sci., Jun 2005; 1044: 94 - 108.

• All Sites:

  Human: Asp175; Mouse: Asp175; Rat: Asp175

Cl-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 3 (cleaved-Asp175) antibody.

Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 3 (cleaved-Asp175) antibody.