Catalog Number:

EGPS001-1, EGPS001-2

Amount:

10ug, 50ug

Other names:

G3PD; GAPD; HEL-S-162eP

Background:

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Expression system:

Human Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) produced in E.coli is a single non-glycosylated polypeptide chain containing 335 amino acids. A fully biologically active molecule has a molecular mass of 37 kDa. The apparent molecular mass of the protein is approximately 40kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques..

Species:

Human

Source:

E. coli

Amino acid sequence:

MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG   IALNDHFVKL  ISWYDNEFGY   SNRVVDLMAH  MASKE

Molecular Weight:

37 kDa, The apparent molecular mass of the protein is approximately 40kD with 6XHis tag observed by reducing SDS-PAGE.

Purity:

> 98% as analyzed by SDS-PAGE.

Formulation:

Purified protein solution after extensive dialysis against PBS, pH 7.5

Endotoxin:

Less than 0.2 EU/μg determined by LAL test.

Storage/Stability:

Samples are stable for up to twelve months from date of receipt and storage at -20℃ to -80℃.

It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.